Extended Data Figure 3: Clonal dynamics are unaffected by AIW surgery.

a, Abdominal imaging windows (AIW) were surgically implanted into the abdominal wall of E-Cadherin-CFP/Lgr5^{eGFP-Ires-CreERT2} mice. To detect CD45⁺ immune cells, tissue sections of the small intestine of these and control (no AIW) mice were stained with CD45 antibodies. Scale bar, 20 µm. b, Quantification of images in a. Regions of interest within the stromal area of the small intestines were measured and the number of CD45⁺ cells was counted within those regions. The bars show averages and s.e.m. (n = 10 regions per mouse performed in 3 mice per condition). c, Five days after tamoxifen injection, the clones of Lgr5^{eGFP-Ires-CreERT2}/R26R-Confetti mice were imaged intravitally. In control mice, the intestine was exteriorized before imaging and, in window mice, a window was placed 2 days before imaging. The frequency of clones with a certain size is plotted in the graph. The lines show the mean and s.e.m. (n = 4 mice per condition).