Extended Data Figure 7: Transient expression of AtSWEET9, SYP41 and RabG2a in leaf epidermis cells of N. benthamiana.
From: Nectar secretion requires sucrose phosphate synthases and the sugar transporter SWEET9
a, Z stacks of confocal images showing the expression of AtSWEET9–GFP proteins, which were driven by a 35S promoter, localized at the plasma membrane and as particles. b, Z stacks of confocal images showing the expression of the trans-Golgi network marker SYP41, fused with enhanced yellow fluorescent protein (eYFP–SYP41) and driven by a 35S promoter. c, Z stacks of confocal images showing the expression of the trans-Golgi network/early endocytotic compartment marker eYFP–RabF2a, which was driven by a 35S promoter. d, e, Co-expression of AtSWEET9–GFP and mCherry–SYP41 driven by a 35S promoter. f, g, Co-expression of AtSWEET9–mCherry and eYFP–RabF2a driven by a 35S promoter. Scale bars, 5 μm.
