Extended Data Figure 5: EC subsets in different skeletal elements and organs.
From: Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone
a–d, Representative tile scan confocal images showing CD31 (green) and Endomucin (red) immunostaining in juvenile (4-week-old) vertebra (a), sternum (b), and whole-mount (c) or sectioned (d) calvarium (parietal bone). Nuclei, DAPI (blue). Arrowheads indicate CD31hiEmcnhi endothelium (yellow). Arrow in a marks an adjacent artery (green). e, Representative dot plots showing flow cytometric analysis of CD31 and Endomucin-stained single cell suspensions from kidney, heart, spleen, lung, brain and liver. Note absence of a CD31hiEmcnhi EC subset (orange dashed circle in Q2) in these organs with exception of liver.
