Extended Data Figure 4: NRROS regulates ROS production and bactericidal activities of phagocytes but not phagocytosis or nitric oxide production by them.
From: NRROS negatively regulates reactive oxygen species during host defence and autoimmunity
a–c, ROS production in IFN-γ-primed BMDMs stimulated with E. coli (a), S. typhimurium (b) and heat-killed L. monocytogenes (HKLM) (c). Averaged AUC from at least four independent experiments is plotted as bar graphs. d, In vitro E. coli killing by neutrophils purified from bone marrow of either WT or KO mice (n = 3). e, Nitric oxide (NO) in culture supernatant of BMDMs stimulated as indicated (n = 3). f, Representative histograms showing phagocytosis of serum-opsonized dsRed-E. coli by BMDMs. Thin line, untreated cells; thick line, treated cells. In the overlay histograms on the right: filled area, WT cells; thick line, NRROS-deficient cells. g, Left, NRROS expression in CD11b+ cells purified from spleens of uninfected WT and L. monocytogenes-infected WT and KO mice. Actin used as loading control. Right, densitometry analyses relative to actin. Error bars, s.e.m. **P < 0.01, ***P < 0.001. Paired (a–c) or unpaired (d, g) Student’s t-test. Data in d–g are representative of at least two independent experiments.
