Extended Data Figure 7: TRPV1⁺ nociceptors regulate IMQ- and DNFB- induced dermatitis and are upstream of IL-23.

a–c, After 3 days of IMQ challenge, ears were harvested and processed for total RNA isolation and Il12b (a), Il23a (b) and Il12a (c) mRNA levels were analysed by qPCR (n = 5). d, DNFB (0.5% in acetone) was applied topically to DMSO and RTX mice. Time course of change in ear thickness of IMQ-treated ear relative to the contralateral ear is represented (n = 10). Two-way ANOVA was run to compare ear swelling under DMSO and RTX conditions over time (****P < 0.0001). e, Representative FACS plots from ears harvested after 24 h of DNFB application. f, IL-23R^GFP/GFP mice were treated with RTX and then compared to wild-type and IL-23R^GFP/GFP littermate controls during IMQ treatment. Ear thickness was calculated relative to the contralateral ear (n = 5). g, After two IL-23 injections into the ear skin of wild-type and IL-23R^GFP/GFP mice, the frequency of IL-17F⁺ cells within dermal γδ T cells was determined by flow cytometry (n = 5). h, IL-23 was injected twice into the ear skin of vehicle- and RTX-treated mice and the total numbers of IL17A⁺ or IL-17F⁺ dermal γδ T cells per ear were determined by flow cytometry (n = 5). NS, not significant.