Extended Data Figure 2: RTX treatment diminishes noxious heat sensation and decreases the expression of nociceptor markers on dorsal root ganglia.

a, Schematic protocol of nociceptor ablation and induction of psoriasiform skin inflammation. RTX was injected subcutaneously into the back in three escalating doses (30 μg kg⁻¹, 70 μg kg⁻¹ and 100 μg kg⁻¹) on consecutive days and mice were allowed to rest for at least 4 weeks before IMQ treatment. b, Denervation was confirmed by immersing the tail of mice into a temperature-controlled water bath maintained at 52 °C and the latency to the first tail movement to avoid water was measured (n = 6). c, Total RNA was isolated from dorsal root ganglia (level C1–C7) of vehicle (DMSO)- and RTX-treated mice and the levels of Trpv1, Scn10a (Na_v1.8), Tac1 (substance P), Mrgprd, Trpm8 and Trpa1 mRNA relative to Gapdh were determined (n = 3).