Extended Data Figure 10: Confirmation of knockdown efficiency by western blot or qPCR.
From: Quantitative flux analysis reveals folate-dependent NADPH production
a, Western blot for G6PD knockdown. b, Western blot for MTHFD1 and MTHFD2 knockdown. c, mRNA level for ME1 knockdown. d, mRNA level for NNT knockdown. e, Western blot for IDH1 and IDH2 knockdown. f, Western blot for ALDH1L2 knockdown. g, Cell doubling times of HEK293T with stable knockdown of indicated genes (results for different hairpins of the same gene were indistinguishable).
