Extended Data Figure 9: Long-term BRL37344 treatment inhibits JAK2(V617F)+ HSC expansion but it does not compromise normal haematopoiesis. | Nature

Extended Data Figure 9: Long-term BRL37344 treatment inhibits JAK2(V617F)+ HSC expansion but it does not compromise normal haematopoiesis.

From: Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms

Extended Data Figure 9

a, b, Haematopoietic progenitors quantitated by FACS (a) or colony-forming units (CFU-C) (b) in BM and blood of WT mice 12-16 weeks after transplantation with MPN (a) and WT BM cells (b), 16 weeks after BRL37344 (2 mg kg−1) or vehicle injections (twice a day, i.p.; n = 3). Mean ± s.e.m. *P < 0.05 (unpaired two-tailed t test). c, BRL37344 treatment does not change normal HSC BM number. CD45.2 WT mice were transplanted with BM cells from congenic CD45.1 WT mice, together with limiting numbers of BM cells from CD45.2 control mice treated with vehicle/BRL37344 over 4 weeks, starting 11 weeks after the transplant (n = 5). The frequency of mice that failed reconstitution 16 weeks after transplantation is plotted against the number of tested cells. HSC frequencies are indicated; n.s., non-significant; Pearson chi-squared t test.

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