Extended Data Figure 4: Neuroglial damage in MPN-affected BM stroma.

a–d, Expression of neuroglial gene sets in MPN BM Nes-GFP⁺ cells. a–c, Selected transcript expression in fragments per kilobase of exon per million fragments mapped (FPKM) of mesenchymal genes (a), HSC-niche related genes (b) and neural-related genes (c). RNA-Seq in CD45⁻CD31⁻Ter119⁻GFP⁺ cells isolated from compound MPN and control mice 6 weeks after pIpC treatment (samples pooled from 3 mice). d, Gene set enrichment analyses (GSEA) of RNA-Seq data. e, f, Enrichment plot of coordinated changes in neuroactive ligand-receptor interactions (e) and the transcriptome of neurons, astrocytes and oligodendrocytes (f). g, h, BM Nes-GFP⁺ cells are different from sympathetic nerve fibres and mature Schwann cells. Immunostaining of tyrosine hydroxylase (TH) to visualize sympathetic nerve fibres (g) and glial fibrillary acidic protein (GFAP) for mature Schwann cells (h) in Nes-gfp BM. Note the close association of Nes-GFP⁺ (green) cells to distinctive TH⁺ or GFAP⁺ (red) cells. i, BM biopsies from (left and middle) control or (right) MPN patients immunostained with (left) secondary Ab as negative control or (middle and right) anti-TH antibodies. Nuclei were counterstained with DAPI (blue). Scale bars, 75 µm (g), 50 µm (h), 100 µm (i).