Extended Data Figure 4: Chromatin 3D interactions at the pdm2 and en loci.
From: Enhancer loops appear stable during development and are associated with paused polymerase
a, c, Reciprocal 4C interaction map (viewpoint, red arrowhead) around the nub and pdm2 (a) or en and inv (c) loci. Top 2 lanes: 4C interactions with a region surrounding the pdm2 or nub (a) and en or inv (c) genes (blue arrowhead). Top lane: 4C using the nub (a) or inv (c) promoter as the viewpoint; bottom lane: using a promoter-proximal region in the vicinity of the pmd2 (a) or en (c) promoter as the viewpoint. Significant 4C interactions and known enhancers are indicated. The pdm2 CE8012 enhancer is indicated. WE, whole embryo. b, d, Expression (double in-situ hybridization) of the pdm2 or inv (green) and nub or en (red) at stage 11, the same stage shown for the 4C interaction in a and c (6–8 h).
