Extended Data Figure 7: Functional and phenotypic characterization of IL2RG-edited T cells harvested from transplanted NSG mice.

a, Graph showing the viability of GFP⁺ and GFP⁻ T cells harvested from the transplanted NSG mice (from Extended Data Fig. 6) or of T cells from peripheral blood of healthy donor (HD T cells), cultured in the presence (+ cyto) or absence (no cyto) of human IL-7 and IL-15. b, Left: representative density plots of GFP⁺ and GFP⁻ T cells harvested from mice, stained for CD8 (left) and CD4 (right). Right: GFP⁺ and GFP⁻ T cells harvested from mice were activated ex vivo with beads coated with anti-CD3 and anti-CD28-specific antibodies, and cultured with IL-7 and IL-15. CD4 and CD8 composition of GFP⁺ and GFP⁻ cells, measured during ex vivo culture, is shown (n = 3). c, Surface phenotype of CD4 and CD8 T cells from b and HD T cells at day 19 after stimulation. A representative plot (left) and histograms with medians + s.e.m. (right) are shown. T stem memory cells (TSCM) are defined as CD62L⁺ CD45RA⁺ (refs 42, 43), T central memory (TCM) as CD62L⁺ CD45RA⁻, T effector memory (TEM) as CD62L⁻ CD45RA⁻ and terminal effectors (TEMRA) as CD62L⁻ CD45RA⁺. d, Production of IL-2 and IFN-γ by GFP⁺ or GFP⁻ T cells as in b, and by HD T cells after 6 h stimulation with PMA+ionomycin. A representative plot (left) and percentages of IL-2⁺ and IFN-γ⁺ cells are shown. P = ns (unpaired t-test). e, IFN-γ Elispot assay showing the frequencies of IFN-γ-producing cells from b challenged with the MDA-MB 231 tumour cell line at different effector to target ratios. PHA stimulation was used as positive control.