Extended Data Figure 6: Actin polymerization-inhibiting agents and the RID domain of Vibrio RTX toxin cannot activate caspase 1 inflammasome.
From: Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome
a, b, Primary BMDMs were left untreated (control), treated with cytochalasin D (Cyto D) or delivered with LFn-tagged actin crosslinking domain (ACD) from Vibrio RTX toxin. c–e, Primary BMDMs or Pyrin-complemented DC2.4 or 293T cells were stimulated with LFn-tagged RID domain from V. cholerae RTX toxin or TcdB. Representative DIC microscopy images of cell morphology are shown in a. Caspase 1 immunoblots are shown in b and c; percentages of cell death measured by LDH release are in d (n = 3; mean ± s.d.). Fluorescence images of RFP–ASC stably expressed in 293T cells are presented in e. Data in all panels are representative of at least three repetitions.
