Extended Data Figure 7: Pyrin inflammasome activation by FIC-domain Rho-adenylylating effectors.
From: Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome
a, b, Effects of Nlrp3, Nlrc4 and Asc knockout on inflammasome activation by FIC-domain Rho-adenylylating effectors. c, d, BMDMs from wild-type mice or two independent Mefv−/− lines (F1-1 and F1-5) were stimulated with various FIC-domain effectors. e, f, Pyrin-complemented DC2.4 cells or primary BMDMs from wild-type or Mefv−/− F1-1 mice were stimulated with indicated toxins or infected with V. parahaemolyticus (V. para) POR3 strain (ΔtdhASΔvcrD2). VopS and IbpA-Fic1/2 were delivered into indicated BMDMs by the LFn-PA system. H/A, mutation of the catalytic histidine. Representative caspase 1 immunoblots from at least three repetitions are shown in a, e and f. ELISA assay of IL-1β release is in b, c, d (n = 3; mean ± s.d.).
