Extended Data Figure 6: Biochemical characterization of Get1/2 transmembrane complexes and proteoliposomes used in Fig. 2b.
From: The Get1/2 transmembrane complex is an endoplasmic-reticulum membrane protein insertase
a, The indicated samples were affinity-purified by anti-Flag immunoprecipitation and elution with Flag peptide, subjected to SDS–PAGE analysis, and visualized by Coomassie blue staining. b, Following proteoliposome reconstitution with the indicated affinity-purified proteins, membranes were separated from the rest of the material by flotation analysis followed by centrifugation of the low-density fraction. The resulting purified proteoliposomes were subjected to SDS–PAGE analysis and visualized by Coomassie blue staining.
