Extended Data Figure 2: Get1/2 TMD mutations used in this study.
From: The Get1/2 transmembrane complex is an endoplasmic-reticulum membrane protein insertase
a, Schematic of Get2-1sc and transmembrane swap mutants used in this study. Ost4 TMD (Nlumen-Ccyto) containing a mutation that abolishes its interaction with other components of the OST complex was used to replace Get1/2 transmembranes that have the same topology. Sec61-β TMD was used to replace the indicated Get1/2 transmembranes of opposite topology. b, ClustalW2 alignment of numbered amino-acid sequences corresponding to the Get2 TM3 (ΔG prediction server version 1.0) from the indicated fungal homologues (Sc, Saccharomyces cerevisiae; Cg, Candida glabrata; Ss, Scheffersomyces stipites; Sp, Schizosaccharomyces pombe) and human calcium-modulating cyclophilin ligand (CAML). Aligned positions were colour-coded by Jalview and the degree of conservation is shown below as a sequence consensus histogram. Asterisk indicates 100% conservation of Get2Sc D271.
