Extended Data Figure 4: Get3 is efficiently recruited to microsomes with a severe genetic disruption of the Get1/2 TMD.
From: The Get1/2 transmembrane complex is an endoplasmic-reticulum membrane protein insertase
a, Whole-cell lysates from the indicated yeast strains were subjected to SDS–PAGE analysis and visualized by immunoblotting. Hexokinase (HXK) was used as a loading control. b, Recombinant Get3–Flag was incubated with the indicated microsomes and analysed by membrane flotation analysis and immunoblotting as in Fig. 2a.
