Extended Data Figure 5: The intracellular domain.
a, View parallel to the membrane plane of the intracellular domain in a surface representation. b, Cross-section of the intracellular domain in a surface representation. c, Cross-section with residues lining the vestibule represented as spheres. d, Cross-section in ribbon, stick and surface representation. e, Two orthogonal views of an obstructed portal in the intracellular domain. The protein is represented as ribbon with sticks for residues lining the narrow tunnel at the portal level. The maximum-likelihood 2mFo−DFc electron density map contoured at a level of 1σ is also represented. Mutations of D417, E42139 and R416, R420 and R42435 all influence the channel conductance. f, Stereo views depicting the position of the three key arginines in the intracellular domain, as seen from the intracellular vestibule and from the pore axis.
