Extended Data Figure 7: mRNA levels of CFP–MS2 under different conditions controlled by various promoter–UTR combinations. | Nature

Extended Data Figure 7: mRNA levels of CFP–MS2 under different conditions controlled by various promoter–UTR combinations.

From: Promoter sequences direct cytoplasmic localization and translation of mRNAs during starvation in yeast

Extended Data Figure 7

a, The relative levels of CFP–MS2 mRNA, under the control of the indicated promoter and UTR regions 15 min after glucose starvation, as measured by qPCR. The values are normalized to ACT1 abundance and are presented as the mean ± s.e.m. relative to the HSP26prUTR-CFP levels and calculated from three independent biological replicates. b, Fold change in CFP–MS2 mRNA abundance after 15 min of glucose starvation (−Glu) or after treatment with 10 mM AZC for 2 h (+AZC), relative to levels during logarithmic-phase growth in glucose-rich medium. CFP–MS2 mRNA was measured by qPCR and normalized to ACT1 levels. The values are presented as the mean ± s.e.m. as calculated from three independent biological replicates.

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