Extended Data Figure 3: Proliferation analysis of Exosc3-deficient B cells.
From: Noncoding RNA transcription targets AID to divergently transcribed loci in B cells
a, FACS analysis indicating the percentage of GFP-negative (left gate) and GFP-positive (right gate) B cells 3.5 days after LPS stimulation. One pair of littermate mice was used. Two biological replicates were performed. b, Kinetic analysis of GFP-positive B-cell accumulation at indicated time points post-LPS stimulation. Indicated Exosc3 genotypes are on a AicdaCre/+ background. One pair of littermate mice was used. Two biological replicates were performed. c, Proliferation analysis determined by VPD450 dilution at 1.5 and 2.5 days post-LPS stimulation. One pair of littermate mice was used. d, Quantitative RT–PCR analysis of Exosc3, Aicda and GFP mRNA expression in GFP+ cells at 3.5 days post-LPS stimulation. Expression levels are normalized to β-actin and plotted relative to Exosc3COIN/+. One pair of littermate mice was used. Data represent mean values from three technical replicates. Error bars represent s.d.
