Extended Data Figure 3: Signal intensity, repair kinetics and residual replication stress in old HSCs.
From: Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells
a, Representative images comparing γH2AX signal intensity in unirradiated young and old HSCs, and 2 Gy irradiated young HSCs. Two different intensity settings for the 488 nm laser are used. b, Additional images for the clearance of γH2AX/53BP1 foci in 2 Gy irradiated young and old HSCs. c, Additional images for γH2AX/RPA and γH2AX/ATRIP staining in young and old HSCs. Scale bars, 10 µm.
