Extended Data Figure 3: δ-cell labelling and tracing in transgenic mice.
From: Diabetes recovery by age-dependent conversion of pancreatic δ-cells into insulin producers
a, The number of Sst+ cells transiently decreases by 80% during the second week after ablation. ncontrol = 255 islets, 7 mice; n3 dpa = 240 islets, 5 mice; n5 dpa = 228 islets, 5 mice; n7 dpa = 251 islets, 5 mice; n0.5 mpa = 267 islets, 6 mice; n1 mpa = 266 islets, 5 mice; n1.5 mpa = 206 islets, 5 mice. Error bars show s.d. Welch’s test (P = 0.0008, 0.0229, 0.006, 0.035), one-way ANOVA (P < 0.0001), Mann–Whitney (P = 0.0043). b, Relative Sst gene expression sharply decreases 2 weeks after β-cell ablation in 2-week-old mice (n = 3 mice per group, each individual sample of each experimental group was run in triplicate, in three independent reactions). Built-in Welch’s test (P = 0.0002). Error bars show s.d. c, Sst-Cre; R26-YFP mice. Cre activity efficiently and specifically occurs in δ-cells (box: enlarged cell). Scale bar, 20 µm. d, Quantitative values of reporter gene expression in islet cells (n = 4; 1,263 YFP+-cells scored).
