Extended Data Figure 10: Degradation of cFLIP in TNF-treated Ripk1−/− MEFs and Ripk1iIEC-KO organoids.
From: RIPK1 ensures intestinal homeostasis by protecting the epithelium against apoptosis
a, Protein lysates were prepared at designated times from Ripk1−/− MEFs treated with mTNF (10 ng ml−1) and analysed by western blot for NF-κB-inducing kinase (NIK), TRAF2, cIAP1 and cFLIP. b, Protein lysates were prepared at designated times from Ripk1iIEC-KO organoids treated with 4-OHT (200 nM) for 20 h before stimulation with mTNF (10 ng ml−1), and analysed by western blot for cIAP1. c, Protein lysates isolated from Ripk1KD-KI organoids were stimulated with mTNF (10 ng ml−1) in an independent experiment and analysed for RIPK1 and cFLIP. WT, wild type.
