Extended Data Figure 7: Analysis of the PfRH5–basigin complex using analytical ultracentrifugation.
From: Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies
a, b, Sedimentation velocity analysis. The continuous sedimentation coefficient distributions that best fit the data are shown for basigin (top), full-length PfRH5 (middle), and a gel-filtered PfRH5–basigin complex (bottom). The inset shows the fitting residuals. c, d, Sedimentation equilibrium analysis. PfRH5ΔN (residues 140–526), basigin, and a gel-filtered PfRH5ΔN–basigin complex were analysed. The runs lasted 20 h at different speeds, as indicated in the inset legends. Ultraviolet absorbance was monitored at 280 nm. The residuals are shown below fitted data. The calculated molecular weights are consistent with the formation of a 1:1 complex between PfRH5ΔN and basigin.
