Extended Data Figure 4: Dietary- and microbiota-associated factors influence the production of pro-IL-1β. | Nature

Extended Data Figure 4: Dietary- and microbiota-associated factors influence the production of pro-IL-1β.

From: Dietary modulation of the microbiome affects autoinflammatory disease

Extended Data Figure 4

a, Footpad homogenates of 12–16-week-old wild-type, LFD-fed Pstpip2cmo and HFD-fed Pstpip2cmo mice were immunoblotted for IL-1β. Data are representative of three independent experiments. b, Footpads samples were collected from 10–14-week-old specific pathogen-free wild-type, germ-free wild-type and Pstpip2cmo × Il1b−/− mice and pro-IL-1β protein levels were determined by western blotting. c, CD45+ cells were isolated from the colons of germ-free wild-type mice and cells were left untreated or stimulated with LPS for 1 h. Relative Il1b mRNA expression levels were determined by qRT–PCR. Two biological replicates, with two technical replicates each.

Back to article page