Extended Data Figure 6: Hi-C analysis of G1-arrested cells.

a, Cohesin (Psc3) localization was examined by immunofluorescence in asynchronous wild-type cells. Cell cycle stage was determined by tubulin staining (TAT1). Psc3 was detected in the nucleus in both G2 and G1/S phase cells (top). Psc3–GFP localization was examined in G1 arrested cells (cdc10-v50). Predominant nuclear staining and Psc3–GFP dots were detected in both asynchronous cells and G1-arrested cells (bottom). Scale bars, 5 μm. b, All-by-all interaction heatmap for G1 cells. The inter-chromosomal cross-like pattern is more prominent in G1 cells than in asynchronous cells. c, 4C-like inter-chromosomal profiles for centromeres and telomeres. d, Global decay of intra-arm contact probability as a function of genomic distance in G1 cells (green) compared with wild type (grey); flat lines indicate average inter-chromosomal contact probability. Slower decay of contact probability over short distances, followed by a more rapid decrease after 100 kb, was observed in G1-arrested cells. The black and grey dashed lines represent the slope for fractal globules (−1) and polymers in a melt (−3/2), respectively. e, FACS analysis of cell populations used for Hi-C (left). Global decay of intra-arm contact probability as a function of genomic distance in G1-arrested rad21-K1 (orange) compared to G1 (green) cells is shown at right. f, Hi-C heatmaps of a segment of chromosome 2 for indicated samples overlaid with lines corresponding to cohesin peaks from the 10-kb binned cohesin (Psc3) profile. The Hi-C directional preference profile is shown below. Note the globules are not visible in G1-arrested rad21-K1 (cdc10-v50 rad21-K1). g, Insulation plot around cohesin peak sites (detected in G1-arrested cells) for G1 and G1-arrested rad21-K1.