Extended Data Figure 7: Globule boundaries in wild-type, rad21-K1, G1-arrested and clr4Δ.
From: Cohesin-dependent globules and heterochromatin shape 3D genome architecture in S. pombe
a, Insulation effect for all cohesin (Psc3) peaks. Relative contact probability was averaged over 20–50 kb at each Psc3 peak region (−50 kb to +50 kb region) and sorted by Psc3 peak height. Insulation effect at Psc3 peaks in wild type, G1 and clr4Δ holds genome-wide, and not only at a small subset of peaks. b, The correlation between Psc3 and relative contact probability Rj(s) profile at a given distance s. The negative correlations hold up to 100 kb in wild type, G1-arrested cells and clr4Δ. Psc3 enrichment and the relative contact probability profile in rad21-K1 do not show an appreciable correlation at any distance. c, The fraction of overlapping boundaries (±1 bin) for each Hi-C data set is shown. Boundaries in clr4Δ and G1-arrested cells show high overlap with wild-type boundaries. The top 100 strongest boundaries from each data set were examined for comparison.
