Extended Data Figure 2: Gastric organoid differentiation is efficient in multiple pluripotent stem cell lines.

a, Table comparing spheroid formation and characteristics between two human ES cell lines (H1 and H9) and one iPS cell line (72.3). Spheroid number was averaged from n = 8 wells per cell line; total cells per spheroid and epithelial composition were determined from whole mount staining (DAPI for total cell number and FOXA2 for epithelial cells) and quantification from n = 6 spheroids per cell line. Error bars represent s.d. b, Immunofluorescent staining of day-34 hGOs derived from ES cell line H1 and iPS cell line 72.3. iPS-cell-derived organoids exhibit the same morphological and molecular features of those derived from ES cells. c, Organ epithelial cell type quantification in day-34 hGOs. Greater than 90% of the epithelium is antral, indicated by PDX1 expression and lack of PTF1A expression, whereas less than 5% express markers associated with other organs derived from endoderm including CDX2 (intestine), albumin (liver) and p63 (squamous epithelium). Data shown are averages from n = 6 hGOs. d–g, Characterization of iPS cell line 72.3 used in a. d, e, iPS cell line 72.3 exhibited normal morphological characteristics of pluripotent stem-cell colonies, as compared to the H1 hESC line (d) and had a normal 46;XY karyotype (e). f, g, iPS cell line 72.3 expressed pluripotent markers OCT3/4 and NANOG (f), and demonstrated pluripotency by differentiation into endoderm, mesoderm, and ectoderm lineages in an in vivo teratoma assay (g). h, Human pluripotent stem-cell scorecard assay results demonstrating that ES cell line H1 and iPS cell line 72.3 have similar pluripotency and differentiation potential, and that iPS cell line 72.3 does not have a lineage bias. EB, differentiated as embryoid bodies for 14 days; UD, undifferentiated. Scale bars, 100 μm. Error bars represent s.d.