Extended Data Figure 5: Interactions between the ADD and CD domains.
From: Structural insight into autoinhibition and histone H3-induced activation of DNMT3A
a, b, GST pull-down assays with recombinant CD (residues 627–912) protein incubated with wild-type or mutant GST–ADD–linker proteins immobilized on glutathione resin. The bound proteins were analysed by SDS–PAGE and Coomassie blue staining. c, GST pull-down assays using wild-type or mutant of the CD domain. d, GST pull-down assays in the absence or presence of histone H3 peptide (H3K4me0 or H3K4me3). e, GST pull-down assays with the CD domain incubated with GST–ADD or GST–ADD–linker proteins immobilized on glutathione resin. f, Activities of wild-type and mutant ADD–CD. Residues 621–632 were replaced by a GS linker in the mutant proteins.
