Extended Data Figure 4: Mrgprb2 is not expressed in mucosal mast cells or peripheral white blood cells.
From: Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions
a, Representative images of a stomach section from an Mrgprb2-tdTomato mouse stained with an anti-MCPT1 (β-chymase) antibody to label mucosal mast cells. White arrows indicate positive cells. No cells were double-labelled (296 Mcpt1-labelled cells and 275 tdTomato-positive cells counted, n = 3 mice). Scale bar, 40 µm. b, Representative images of a Cytospin preparation of peripheral white blood cells from an Mrgprb2-tdTomato mouse doubly labelled with tdTomato for Mrgprb2-expressing cells (red; left image) and Hoechst 33342 nuclear staining (blue; right image). No peripheral white blood cell expressed tdTomato (n = 3 mice; >4,000 cells examined). Scale bar, 40 µm.
