Extended Data Figure 5: Mrgprb2MUT mice are functional knockouts.
From: Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions
a, Illustration of the genomic region in and around the Mrgprb2 locus. Note that repetitive sequences including long interspersed elements (LINEs), short interspersed elements (SINEs), and long tandem repeats (LTRs) begin immediately after the 3′ side of the Mrgprb2 gene, and in addition are present within 2.5 kb of the 5′ side. A BLASTN search in March 2014 using the 500 bases adjacent to the 3′ end of Mrgprb2 as a query turned up more than 269,000 hits in the mouse genome. b, Comparison of the wild-type (WT) and mutant (MUT) genomic sequences shows the location of the 4 bp deletion in the mutant. Numbers correspond to the Mrgprb2 open reading frame. c, Sequencing result from wild-type and mutant complementary DNA sampled from mice born 18 months after the mutant line was established. The bases missing in the mutant are highlighted in red. d, Amino acid translation of the Mrgprb2MUT open reading frame reveals that the deletion creates a frameshift mutation and an early termination codon (marked with an asterisk) shortly after the first transmembrane region. Mut, site of the frameshift deletion; TM1, transmembrane region 1.
