Extended Data Figure 7: PLK1 was identified as a MEIKIN interactor.
From: Meikin is a conserved regulator of meiosis-I-specific kinetochore function
a, Yeast two-hybrid screening of mouse MEIKIN interactors was performed using a mouse testis cDNA library. The number of clones isolated by screening is summarized. Because the use of MEIKIN full-length and N-terminal region (amino acids 1–271) as bait resulted in a high background of false positive interactions in yeast two-hybrid screening, we used MEIKIN C terminus (amino acids 272–434) as bait. b, Yeast two-hybrid assay demonstrates that MEIKIN interacts directly with mouse PLK1 through the MEIKIN C-terminal domain (amino acids 272–434). c, Mouse MEIKIN protein was immunoprecipitated from testis chromatin-bound fraction by two different anti-MEIKIN polyclonal antibodies (A and B). The immunoprecipitates underwent two independent LC–MS/MS analyses. Those proteins commonly identified in all the LC–MS/MS analyses are listed with the number of peptide hits in the table. Note that polo-like kinase (PLK1) as well as CENP-C were repeatedly identified in the two-hybrid screening (a) and LC–MS/MS analyses (c).
