Extended Data Figure 9: The human homologue of MEIKIN.
From: Meikin is a conserved regulator of meiosis-I-specific kinetochore function
a, Schematic illustrations of mouse and human MEIKINs. The putative amino acid sequence of hMEIKIN full-length (373 amino acids) was deduced from our own sequencing of DNA (see Extended Data Fig. 2), which was amplified by RT–PCR from the human testis cDNA. The amino acid sequence equivalent to mouse exon 10 (mEX10) is absent in the hMEIKIN protein, despite our attempts at a computational search to identify the missing DNA sequence. b, Yeast two-hybrid assays demonstrate that the hMEIKIN C terminus interacts with human CENP-C (full length), hCENP-C C-terminal (CENPC motif + Mif2 motif, amino acids 732–945) and hPLK1, in agreement with mouse MEIKIN data. c, Immunostaining of a human seminiferous tubule section (purchased from Biochain) demonstrates that hMEIKIN localizes to centromeres (ACA) in pachytene spermatocytes. We used anti-hMEIKIN-N and anti-hMEIKIN-C antibodies. Enlarged images of the rectangles are shown to highlight the co-localization of ACA and hMEIKIN (bottom). Scale bar, 5 μm.
