Extended Data Figure 1: MEIKIN (4930404A10rik) was identified as a meiosis-specific CENP-C binding protein.
From: Meikin is a conserved regulator of meiosis-I-specific kinetochore function
a, Yeast two-hybrid screening was performed using CENP-C C terminus (amino acids 692–906) as bait and a mouse testis cDNA library as prey. A total of 11.95 × 107 colonies were screened on selective (SD-Trp-Leu-His-Ade, +10 mM 3AT) plates using the AH109 tester strain. The number of clones isolated by screening is summarized. b, To search for a meiosis-specific candidate from the isolated clones, tissue-specific expression patterns of CENP-C interactors were examined by RT–PCR. RNA was extracted from each tissue of both males and females. Testis RNA was derived from 8-week-old males. Ovary RNA was derived from 4- and 8-week-old females. RT(−) indicates control PCR without reverse transcription. Note that the expression of 4930404A10rik is restricted to testis and ovary, as is that of SMC1β. c, Immunoprecipitates from mouse testis chromatin extracts using anti-CENP-C antibody or control IgG were analysed by the indicated antibody. d, The C-terminal domain of MEIKIN (4930404A10rik) (amino acids 385–434) interacts with the CENP-C C terminus in yeast two-hybrid assay.
