Extended Data Figure 2: Sequence alignment of MEIKIN homologues in vertebrates.
From: Meikin is a conserved regulator of meiosis-I-specific kinetochore function
Amino acid sequences of M. musculus 4930404A10Rik (NP_083381), R. norvegicus (XP_573090), C. lupus (XP_003639413), X. tropicalis (XP_002934413) and G. gallus (XP_001234011) are derived from the NCBI protein database. H. sapiens data are derived from cDNA clones. 4930404A10Rik protein is conserved among vertebrates but it does not have any known motif except for the polo-box binding motif (blue line) in mammalian proteins. Two-hybrid assays indicate that this motif of mouse MEIKIN is important for PLK1 binding (data not shown), although the motif is apparently not conserved in Xenopus and chicken. The C-terminal sequences (red lines) are required for the kinetochore localization (see Fig. 1c).
