Extended Data Figure 3: Co-localization of CENP-C and ACA in spermatocytes and MEKIN localization in oocytes.
From: Meikin is a conserved regulator of meiosis-I-specific kinetochore function
a, Squashed spermatocytes from wild-type were immunostained for CENP-C, ACA, SYCP3 and DAPI at the indicated stages during meiosis. CENP-C and ACA signals accumulate and co-localize at centromeres after zygotene throughout meiosis. b, Chromosome spreads of oocytes from wild-type were immunostained for MEIKIN, ACA and REC8 at different meiotic stages. Zygotene from E15.5, pachytene and diplotene from E18.5 mice. c, Chromosome spreads of oocytes at metaphase I (5 h after GVBD) and metaphase II (16 h post GVBD) were stained for MEIKIN, ACA and DAPI. Scale bars, 5 μm.
