Extended Data Figure 1: Overview and graphical summary of this study.
From: X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis
a, b, Final stages of teicoplanin aglycone biosynthesis (a) and vancomycin-type aglycone biosynthesis (b). c, d, In vitro binding studies demonstrated that the minimal interaction interface of OxyA–C is the conserved X-domain from the final module of the teicoplanin non-ribosomal peptide synthetase (c) and that the OxyBtei and OxyAtei enzymes required for peptide maturation are only active against peptide-bound PCP7-substrates that also contain this X-domain (d). These results support the hypothesis that in vivo aglycone formation occurs through the interaction of Oxy enzymes with module seven of the teicoplanin non-ribosomal peptide synthetase that then act upon the NRPS-bound linear heptapeptide precursor.
