Extended Data Figure 2: Interaction of Oxy enzymes with NRPS domains studied by analytical size-exclusion chromatography and multi-angle light scattering. | Nature

Extended Data Figure 2: Interaction of Oxy enzymes with NRPS domains studied by analytical size-exclusion chromatography and multi-angle light scattering.

From: X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis

Extended Data Figure 2

a, b, Profiles of OxyBtei (red) shown with the profiles of the corresponding NRPS domain constructs and variants (orange) and mixtures of the binding partners in a 1:3 ratio (blue). c, Profiles of OxyAtei, OxyCtei, OxyEtei (red) shown with the profiles of the corresponding GB1–X constructs (orange) and mixtures of the binding partners in a 1:3 ratio (blue). Solid lines represent detection at 415 nm and dashed lines at 280 nm detection.

Back to article page