Extended Data Figure 3: Interaction of Oxy enzymes with NRPS domains studied by native-PAGE.
From: X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis
a, Top, overview of electrophoretic migration behaviour of OxyBvan and OxyBtei in the absence/presence of different NRPS domain constructs (GB1–PCP7, –PCP7–X, –PCP7–X–TE, –X; 1:3 molar ratio). Bottom, interaction studies of OxyBtei with NRPScep proteins and OxyBvan with NRPStei proteins. b, Overview of electrophoretic migration behaviour of OxyAtei, Btei and Ctei in the absence/presence of GB1–Xtei or GB1–PCP7–X–TEtei (1:3). c, Titration of OxyBtei (10 µM) with GB1–Xtei (0 to 30 µM) leading to the appearance of a new band with low electrophoretic mobility and the disappearance of free OxyBtei at equimolar concentrations. The bands corresponding to an Oxy/X complex are marked with an asterisk, those corresponding to an Oxy/PCP7–X–TE complex with a triangle and those corresponding to Oxy/PCP7–X with a square.
