Extended Data Figure 7: Relation of Notum to other esterases of the α/β hydrolase family.
From: Notum deacylates Wnt proteins to suppress signalling activity
a, Comparison between hNOTUM and human esterase D (hESTD), showing structural relatedness. hNOTUM is also related to hAPT1, a cytosolic esterase used in this study as a positive control for fatty acid esterase activity. In the views shown here, the hNOTUM structure has been rotated by 90° around the x axis relative to the structure shown in Fig. 3b. b, Rootless phylogenetic tree of animal Notum proteins (red) and plant pectin acetylesterases (PAE, green). Extent of sequence identity to hNOTUM is shown next to species name. Percentages between branches indicate sequence identity between neighbours.
