Extended Data Figure 6: Characterization of the grnd^Minos mutant.

a, Schematic representation of the Minos transposon insertion in the grnd gene region corresponding to the 5′ untranslated region of the transcript. b, Western blot of wing disc extracts from the indicated genotypes probed with anti-Grnd (top) and anti-β-tubulin (bottom) antibodies. Grnd protein migrates at the predicted size of 27 kDa (lane 1, control). Grnd protein levels are decreased below detection level in both grnd^Minos/Minos and grnd^Minos/Df mutant flies, indicating that grnd^Minos/Minos is indistinguishable from a null mutant. c, d, Polarity is not affected in grnd^Minos/Minos and grnd^Minos/Df wing discs. Transverse sections of dissected control (c) or grnd^Minos/Minos (d) wing discs stained for Grnd (top; red), Crb (middle top; blue) and Dlg (middle bottom; green). e–j, Wing discs dissected from third instar larvae after 40 h of Egr expression in the rn domain. Pouch ablation, ubiquitous Wg expression and apoptosis observed in rn>egr, Tub-Gal80^ts discs (e) are suppressed to various extents upon co-expression of grnd-RNAi (f) or reduction of JNK activity (g, h) and fully suppressed in grnd^Minos/Minos (i) and grnd^Minos/Df (j) mutant backgrounds.