Extended Data Figure 7: Grnd is highly expressed in RasV12/scrib−/− micro-metastases.
From: The Drosophila TNF receptor Grindelwald couples loss of cell polarity and neoplastic growth
a–c, Reducing grnd levels in scrib−/− mutant clones prevent their elimination. Eye discs dissected 5 days AED carrying GFP-labelled MARCM clones of the indicated genotypes stained for Grnd (middle; red) and DAPI (right; grey). d, Reducing Grnd levels does not affect cell viability. Eye discs dissected 5 days AED carrying GFP-labelled grnd RNAi Flip-out clones stained for Grnd (middle; red) and DAPI (right; grey). e–g, Invasive brain tumours and micro-metastases display high levels of Grnd protein. Dissected eye–brain complex (e), gut (f) and fat body (g) displaying GFP-labelled invasive RasV12/scrib−/− clones (e; green) or micro-metastases (f, g; green) stained for Grnd (red) and DAPI (blue).
