Extended Data Figure 8: Grnd, but not Wgn, is required for Mmp1 expression both in RasV12/scrib1 and RasV12/Dlg RNAi tumours.
From: The Drosophila TNF receptor Grindelwald couples loss of cell polarity and neoplastic growth
a–c, Eye–brain complexes dissected 7 days AED carrying RasV12/scrib−/− (a), RasV12/scrib−/− + grnd-RNAi (b), or RasV12/scrib−/− + dilp8-RNAi (c; control RNAi to exclude a Gal4 titration effect) clones labelled by GFP and stained for Mmp1 (middle; red). RasV12/scrib−/− and RasV12/scrib−/− + dilp8-RNAi clones invade the ventral nerve cord (a, c; see white arrow). d–g, Eye–brain complexes dissected 7 days AED carrying RasV12/Dlg-RNAi (d) or RasV12/Dlg-RNAi + grnd-RNAi (e, f) clones (labelled by GFP), or RasV12/Dlg-RNAi GFP-labelled clones in a wgn-null mutant background (g) stained for Mmp1 (middle; red) and DAPI (right; blue). RasV12/Dlg RNAi clones invade the ventral nerve cord in wgn-null mutant animals (g; see white arrow).
