Extended Data Figure 7: Contribution of DAXX, ATRX, KAP1 and ESET to H3.3 enrichment at ERVs.
From: Histone H3.3 is required for endogenous retroviral element silencing in embryonic stem cells
Related to Fig. 2. a–d, ChIP‐qPCR analysis of H3.3 enrichment at various repeat regions in control and ATRX‐knockout (a), DAXX‐knockout (b), KAP1‐knockout (c) and ESET‐knockout (d) ES cells. Error bars represent s.d. from one experiment (n = 3). Data are representative of two independent ChIP experiments. e, ChIP‐seq enrichment of KAP1 and H3.3 in control and KAP1‐knockout ES cells at repetitive regions of the mouse genome. Data are represented in a heat map of log2 fold enrichment (red) or depletion (blue) over a matched input. f, Loss of H3.3 reduces nuclear DAXX levels. Immunoblot from whole‐cell extracts (WCE) or nuclear extracts (NE) in the presence and absence of H3.3. Asterisk denotes cross‐reacting band. g, ChIP‐seq enrichment of KAP1 and DAXX in control and H3.3‐knockout ES cells. Data are represented as in e. Note the different colour scale used for KAP1 and DAXX.
