Extended Data Figure 5: H3.3 at IAPs is not associated with transcription, DNase I or MNase sensitivity.
From: Histone H3.3 is required for endogenous retroviral element silencing in embryonic stem cells
Related to Fig. 1. a, Direct comparison of chromatin properties at TSSs of highly expressed genes and IAP ERVs. Box plots showing (from left to right) comparable enrichment of H3.3; DNase I sensitivity; MNase sensitivity; elongating RNAP2 occupancy. MNase data sets are from a recent study, showing H3.3 localizing to MNase hypersentitive regions such as active promoters25. In this study, MNase sensitivity was assessed by sequencing nucleosomes released under mild (‘short’) or extensive (‘long’) MNase digestion conditions; MNase hypersensitive sites were shown to be specifically enriched by mild MNase digestion, whereas long digestion released chromatin more evenly25. b, Comparison of kinetics of H3.3 incorporation26 at the TSS of highly expressed genes and IAP ERVs; as control, a randomized set of intervals is shown.
