Extended Data Figure 7: Active β-catenin signalling blocks CCL4 production in human melanoma cell lines.
From: Melanoma-intrinsic β-catenin signalling prevents anti-tumour immunity
a, Western blot on mel537 and mel888 showing stabilized β-catenin expression. b, Expression level of human ATF3 and human CCL4 in mel537 and mel888 (three independent experiments, duplicates per experiment). c, Expression level of β-catenin target genes in mel537 and mel888. d, ATF3-specific ChIP assay in mel537 and mel888 cell lines for the CCL4 gene locus (two independent experiments, duplicates per experiment). e, CCL4 secretion (left) and ATF3 transcription levels (right) after siRNA-mediated knockdown of CTNNB1 and ATF3 in mel537 and mel888 assessed by ELISA or qRT–PCR, respectively (two independent experiments, duplicates per experiment). All data are mean ± s.e.m., Mann–Whitney U test. *P ≤ 0.05.
