Extended Data Figure 3: T-cell infiltration of genetically engineered mice.
From: Melanoma-intrinsic β-catenin signalling prevents anti-tumour immunity
a, Representative images of immmunofluorescent staining against CD3 (red, left panel) and TRP1 (green, right panel) in all three tumour tissues (scale bar, 100 μm; ×4, ×10, ×20 with ×4 differential interference contrast (DIC) on top; nuclei Hoechst ×20 CD3 stain as shown in Fig. 1). b, Representative immmunofluorescent staining against CD3 (red, left panel) and TRP1 (green, right panel) in a highly pigmented area of BrafV600E/Pten−/− tumour tissues (scale bar, 100 μm; ×10, ×20 with ×10 DIC left) excluding that the lack of T cells is associated with increased pigmentation (nuclei Hoechst). c, Numbers of CD3+ T cells were counted within 13 different fields (0.5 mm × 1 mm) from two tumour samples. Mean of 12 T cells or 3.2 T cells per 0.5 mm2 in BrafV600E/CAT-STA or BrafV600E/Pten−/−/CAT-STA tumours, respectively, versus 100 T cells per 0.5 mm2 in BrafV600E/Pten−/− tumours. Data are given as mean with minimum and maximum, as well as individual values. Statistical analysis was performed using Mann–Whitney U test. ****P ≤ 0.0001.
