Extended Data Figure 7: Meningeal lymphatic vessels carrying immune cells.

a, Representative images of T cells (CD3e) and lymphatic endothelial cells (Lyve-1) on dural sinuses (scale bar, 20µm). a_ii–a_iii, Orthogonal sections representing T-cell localization around a_ii and within a_iii the Lyve-1 structures (scale bars, 5µm). b, Quantification of the sinusal T cells and MHCII-expressing cells within the lymphatic vessels (mean ± s.e.m., n = 7–8 mice from 3 independent experiments). c, d, Representative images of Lyve-1 staining on dural meninges from CD11c^YFP mice (scale bars, 20 µm). CD11c-positive cells (most probably dendritic cells) can be found inside the meningeal lymphatic vessels. e, Representative image of B220⁺ cells and lymphatic endothelial cells (Lyve-1) immunolabelling in the meninges (yellow arrowheads indicate B220⁺CD11c^– cells; scale bar, 20 µm). f, Representative dot plots of B220⁺ cells (gated on singlets, live, CD45⁺) within the dural sinuses expressing CD19; ∼40% of the B220⁺ cells express CD19.