Extended Data Figure 2: Identification, characterization and validation of the expression of classical lymphatic endothelial cell markers by the meningeal lymphatic vessels.
From: Structural and functional features of central nervous system lymphatic vessels
a, Representative images of Prox1 labelling on meningeal Lyve-1 expressing vessels (n = 3 mice; scale bars, 10 µm). b, Schematic representation of the whole-mount dissection of the diaphragm. c, Characterization of the specificity of the podoplanin antibody. Representative images of whole-mount diaphragm labelled with anti-Lyve-1 and anti-podoplanin (ci), control isotype (cii) or the anti-podoplanin pre-incubated overnight with a saturated concentration of recombinant podoplanin protein (ciii; scale bars, 20 µm). d, Characterization of the specificity of the VEGFR3 antibody. Representative images of whole-mount diaphragm and dura mater labelled with anti-Lyve-1 and anti-VEGFR3 (di), secondary antibody only (dii), or the anti-VEGFR3 pre-incubated overnight with a saturated concentration of recombinant VEGFR3 protein (diii; scale bars, 20 µm). e, Quantification of the number of Prox1+ nuclei per mm2 of lymphatic vessel (mean ± s.e.m.; n = 4 animals each group).
