Extended Data Figure 8: SHARP, LBR, SAF-A, SMRT, and HDAC3 are required for transcriptional silencing in differentiating female ES cells.
From: The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3
a, Representative images showing staining of DAPI (blue), Xist (red), and Gpc4 (green) upon knockdown of specific proteins using different siRNAs in female ES cells before differentiation (−RA; left) or after differentiation for 24 h (+RA; middle and right). RA, retinoic acid. b, Quantification of the copy number of Gpc4 for –RA and +RA cells upon transfection with different siRNAs. Error bars represent the standard error of the mean across 50 individual cells from one experiment. NS, not significantly different between +RA and –RA cells; ****P value < 0.001, **P value < 0.01, and *P value < 0.05 between +RA and –RA cells based on an unpaired two-sample t-test. Scale bars on the images represent 5 μm.
