Extended Data Figure 5: RT–PCR confirmation of RS-sites in human and zebrafish samples, and prediction of mouse RS-exons. | Nature

Extended Data Figure 5: RT–PCR confirmation of RS-sites in human and zebrafish samples, and prediction of mouse RS-exons.

From: Recursive splicing in long vertebrate genes

Extended Data Figure 5

a, Schematic of primer design used for RT–PCR validation of novel junctions. b–g, RT–PCR analysis of CADM2 (b), HS6ST3 (c), ROBO2 (d), PDE4D_1_1 (e), PDE4D_1_2 (f) and PDE4D_2_2 (g) genes around RS-sites using indicated primers. For PDE4D sites, first number after gene name indicates RS-site studied, second number indicates the upstream exon used. See Extended Data Fig. 3g for junctions detected. h, RT–PCR analysis of cadm2a RS-site junction in adult male and female zebrafish embryos, together with an alignment of zebrafish (ZF) cadm2a RS-site to human (HS) CADM2 RS-site. i, Map of consensus splice site location and in-frame termination codons following RS-sites in indicated mouse genes. Strong consensus splice sites are GTAAG, GTGAG, GTAGG and GTATG. Weak consensus splice sites are GTAAA, GTAAT, GTGGG, GTAAC, GTCAG and GTACG.

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